Cell Line Authentication and Testing

If you need to authenticate your cell line for an NIH grant, or if you are looking to satisfy your IACUC prior to clearance of your transplantation studies, contact CH3 BioSystems. You can even design your own test panel! We have a broad array of options for you (see below). In addition, species-specific PCR evaluation … Read more

Do you have questions about mouse E0771 cells?

Recently there has been some confusion on the internet about the proper authentication of the mouse E0771 cell line. CH3 BioSystems is the longest-running supplier of authentic E0771 cells to both academic and commercial laboratories. CH3 BioSystems acquired an isolate of E0771 cells in 2011 in a contractual arrangement with a world class NCI-designated Comprehensive … Read more

Happy New Year From CH3 BioSystems!

MANY THANKS AND HAPPY NEW YEAR TO CH3 BIOSYSTEMS’ ACADEMIC AND CORPORATE CUSTOMERS ALL OVER THE WORLD. We wish everyone peace, good health and success for your research projects in 2021! You can always rely on CH3 to be here to provide you with: High Quality Unique Cell Lines Customer Service Expertise for managing international … Read more

SMAD6 Methylation Reduces Periodontal Inflammation

Continuing last week’s post about protein methylation in cellular inflammatory responses, we would like to call attention to the recent publication by Zhang et al. The authors explain how PRMT1-SMAD6 signaling may represent a new target for reducing the immune response in periodontitis (see Figure): Lipopolysacchride (LPS) from oral pathogens activate toll-like receptors (TLR) which … Read more

Protein Methylation in Cellular Inflammatory Responses

We are thinking a lot lately about the role of protein methylation in cellular inflammatory responses. The connection between the two is not a new idea. There are numerous clues in the primary scientific literature that functionally link protein methylation with inflammation. See for example the recent review by Webb and Guerau-de-Arellano There will be … Read more

New Analysis of Protein Arginine Methylation in Colorectal Cancer

This week’s blog post highlights a recent study by Lim et al. This proteome-wide analysis of protein arginine methylation in colorectal cancer based on human surgical specimens is another example of the developments occurring in our field with real impact on disease diagnosis. For histological detection of the methylation status of colon cancer, we recommend … Read more

Important Translational Research News About Fragile X Mental Retardation and Cancer

CH3 BioSystems is excited about an important review article recently published by Majumder et al. that helps to explain the role of methylarginine modification in the functional regulation the fragile X family of proteins with implications for neurodevelopment and cancer. In conjunction with phospho-serine modifications of these methyl-proteins, the molecular mechanisms involved are likely to … Read more

New Discoveries in Protein Methylation

A ground-breaking report from scientists at Glaxo and Epizyme has now revealed that the PRMT1 inhibitor, GSK3368715, exhibits potent anti-tumor activity. Extensive chemical and cellular studies support the usefulness of the new chemical compound as a First-In-Class therapeutic agent. Also described in the report are experiments that implicate the endogenous metabolic inhibitor, methyl-thioadenosine (MTA), in … Read more

Current Research Using the E0771 Cell Line

The use of E0771 cells in cancer metastasis research is rapidly increasing in popularity. Researchers are attracted to the aggressive nature and immune system evading properties of the cells. One of the main interests of researchers using these cells is the role of immune cell antitumor functions in the regulation of cancer spread. Immune Responses … Read more

Using E0771 Cells for Allograft Transplantation

After removing E0771 cells from cell culture using sterile technique, the cells are counted and injected into a healthy mouse via the mammary pads (typically the fourth mammary pad) or intravenously in the tail vein [1-8]. To see the full effects of tumor growth, 1 x 105 to 1 x 106 must be used for … Read more