New Analysis of Protein Arginine Methylation in Colorectal Cancer

This week’s blog post highlights a recent study by Lim et al. This proteome-wide analysis of protein arginine methylation in colorectal cancer based on human surgical specimens is another example of the developments occurring in our field with real impact on disease diagnosis. For histological detection of the methylation status of colon cancer, we recommend … Read more

Important Translational Research News About Fragile X Mental Retardation and Cancer

CH3 BioSystems is excited about an important review article recently published by Majumder et al. that helps to explain the role of methylarginine modification in the functional regulation the fragile X family of proteins with implications for neurodevelopment and cancer. In conjunction with phospho-serine modifications of these methyl-proteins, the molecular mechanisms involved are likely to … Read more

New Discoveries in Protein Methylation

A ground-breaking report from scientists at Glaxo and Epizyme has now revealed that the PRMT1 inhibitor, GSK3368715, exhibits potent anti-tumor activity. Extensive chemical and cellular studies support the usefulness of the new chemical compound as a First-In-Class therapeutic agent. Also described in the report are experiments that implicate the endogenous metabolic inhibitor, methyl-thioadenosine (MTA), in … Read more

Current Research Using the E0771 Cell Line

The use of E0771 cells in cancer metastasis research is rapidly increasing in popularity. Researchers are attracted to the aggressive nature and immune system evading properties of the cells. One of the main interests of researchers using these cells is the role of immune cell antitumor functions in the regulation of cancer spread. Immune Responses … Read more

Using E0771 Cells for Allograft Transplantation

After removing E0771 cells from cell culture using sterile technique, the cells are counted and injected into a healthy mouse via the mammary pads (typically the fourth mammary pad) or intravenously in the tail vein [1-8]. To see the full effects of tumor growth, 1 x 105 to 1 x 106 must be used for … Read more

Introduction to the E0771 Cell Line

E0771 is a mouse breast adenocarcinoma cell line. The original source of E0771 cells was a spontaneously occurring breast tumor from C57BL/6 mouse [1]. The tumor was removed from the breast fat pad of the mouse and adapted for anti‐cancer drug testing by Sirotnak et al. in 1984 [2]. Cell culture conditions as described in … Read more

Research Review Series (2 of 3)

THE INTERSECTION OF CANCER AND PROTEIN METHYLATION   William Pratt, CH3 Biosystems Lab Intern Introduction   Protein arginine methyltransferases (PRMTs) are transcriptional coactivators, a type of coregulator that affects a transcription factor to increase the rate of transcription of a gene by recruiting general transcription machinery to the promoter region. The aberrant expression of PRMTs … Read more

Research Review Series (1 of 3)

PRMT5 and Cancer  William Pratt, CH3 Biosystems Lab Intern   Protein methylation is a common post translational modification that has grown in importance as a research topic in recent years. The role of protein methylation in cancer is extremely complicated and thus very difficult to discuss broadly. This series of posts focuses on the relationship … Read more

Product Spotlight

PC12 Pheochromocytoma Cells     Advantage of PC12 Cells Every year thousands of people in the world are diagnosed with neurodevelopmental diseases and cancer. The need for better and more effective medications that can be brought to the market faster is on the rise. Each new drug is created from years of research and experiments … Read more