After removing E0771 cells from cell culture using sterile technique, the cells are counted and injected into a healthy mouse via the mammary pads (typically the fourth mammary pad) or intravenously in the tail vein [1-8]. To see the full effects of tumor growth, 1 x 105 to 1 x 106 must be used for injection. Cells are injected when the mice reach 7-8 weeks old .
Primary tumors begin to form within days but must grow for about two weeks before observing metastasis .
When you order E0771 cells, CH3 Biosystems always provides 10 to 20 x 106 cells in the vial or flask that is shipped to you. This makes it easier to get your experiment up and running without taking extra time to grow the cells!
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– Written by Nicole Cavanaugh
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