PC12 Cell Line
Rat pheochromocytoma clonal cell line. The original parental line isolated in 1976 by Greene and Tischler (https://www.ncbi.nlm.nih.gov/pubmed/1065897). Neuronal differentiation induced by nerve growth factor.
Re-sale or other commercial use of CH3 PC12 Cell Lines requires the execution of a Licensing Agreement, Materials Transfer Agreement, or other codified accord between CH3 BioSystems LLC and the commercial, for profit organization.
40 to 80 X 106 cells in flask containing recommended culture medium or frozen cell pellet in cryoprotectant.
Cell culture. Use RPMI 1640 medium; 10% Horse Serum; 5% Fetal Bovine Serum. For neuronal differentiation, use 1% Horse Serum + 50 ng/ml nerve growth factor.
Proliferation (4 day doubling time) and differentiation tested on a substrate of rat-tail collagen.
Place in culture immediately upon arrival; 35° humidified CO2 incubator. Use 5 to 7.5% CO2.