Serine Protease Inhibitor
Convenient ready-to-use protease inhibitor compatible with in vitro arginine methyltransferase reactions. Dilute 4 μl/ml of pre-warmed (~55°) assay buffer; vortex and cool immediately in wet ice prior to addition of enzyme, substrate and S-Adenosylmethionine for methyltransferase assay.
For use during in vitro methylation of recombinant or native proteins.
Validated by in vitro methylation using CH3 Arginine-Rich Peptide.
Thaw one tube for single use only. Unstable at room temperature. Store remaining aliquots at -80°. Stable for 3 months after the date of preparation printed on the label.