E0771 Cell Line
E0771 murine breast cancer cell line
Medullary breast adenocarcinoma cell line originally isolated as a spontaneous tumor from C57BL/6 mouse (Sugiura, K and Stock, C.C., 1952) and adapted for anti‐cancer drug testing by Sirotnak et al., 1984 ( https://www.ncbi.nlm.nih.gov/pubmed/6690070 ). Cell culture conditions as described by Ewens et al., 2005 (https://www.ncbi.nlm.nih.gov/pubmed/16312045) allow maintenance as a cell monolayer.
10 to 20 X 10^6 cells provided in tissue culture flask or as a frozen cell pellet in cryoprotectant.
Cell culture. RPMI 1640 medium supplemented with 10 mmol/L HEPES and 5% to 10% Fetal Bovine Serum. Use of antibiotics, such as penicillin (100 units/ml) and streptomycin (100 μg/ml) is not required. The decision to use antibiotics is left to the discretion of the investigator.
Cultured on tissue culture plastic in 5% fetal bovine serum with either DMEM or RPMI 1640.
Place in culture immediately upon arrival; 37° humidified (100%) CO2 incubator. Use 5% CO2.