E0771 Breast Cancer Cell Line

Description

E0771 Cell Line – Murine Breast Cancer Cell Line

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Product: #94A001

Overview

Medullary breast adenocarcinoma cell line originally isolated as a spontaneous tumor from C57BL/6 mouse (Sugiura, K and Stock, C.C., 1952) and adapted for anti‐cancer drug testing by Sirotnak et al., 1984 ( https://www.ncbi.nlm.nih.gov/pubmed/6690070 ). Cell culture conditions as described by Ewens et al., 2005 (https://www.ncbi.nlm.nih.gov/pubmed/16312045) allow maintenance as a cell monolayer.

Purification

N/A

Formulation

10 to 20 X 10^6 cells provided in tissue culture flask or as a frozen cell pellet in cryoprotectant.

Product Usage

Cell culture. RPMI 1640 medium supplemented with 10 mmol/L HEPES and 5% to 10% Fetal Bovine Serum. Use of antibiotics, such as penicillin (100 units/ml) and streptomycin (100 μg/ml) is not required. The decision to use antibiotics is left to the discretion of the investigator.

Testing

Cultured on tissue culture plastic in 5% fetal bovine serum with either DMEM or RPMI 1640.

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Selected Lots of the E0771 cell line are authenticated by short tandem repeat profiling and screened for the following pathogens:

Pathogen Testing of CH3 Biosystems Breast Cancer Cell Line

(M. spp., M. pulmonis) – Mycoplasma
(TMEV) – Theiler’s Murine Encephalomyelitis Virus
(PVM) – Pneumonia Virus of Mice
(MVM) – Minute Virus of Mice
(MHV) – Mouse Hepatitis Virus
(MPV1-5) – Mouse Parvovirus
(C. bovis) – Corynebacterium bovis, Sendai Virus

In addition, species-specific PCR evaluation is performed to rule out any contamination by rat, human, hamster or monkey cells.

Screening for additional pathogens may be available upon request. Contact us for customized pricing.

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Storage

Place in culture immediately upon arrival; 37° humidified (100%) CO2 incubator. Use 5% CO2.