CH3 Enzymes

20 mM Tris pH 8

$20.00

PRMT7 is a unique recombinant type III protein arginine methyltransferase expressed as a fusion protein in bacteria. As supplied, the enzyme is extremely active (https://www.ncbi.nlm.nih.gov/pubmed/19110445) and capable of mono-methylation of arginine acceptor sites in peptides and proteins under appropriate incubation conditions.

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Unit Size:

100 ml

SKU: 840001 Category:

20 mM Tris pH 8

Low Ionic Strength Reaction Buffer

Product: #840001

Overview

Convenient ready-to-use basic buffer compatible with in vitro arginine methyltransferase reactions. Prepared from equimolar ACS grade Tris HCl and Tris Base to produce pH = 8.

Purification

N/A

Formulation

Prepared with de-ionized water (≥18 MΩcm).

Product Usage

For use during in vitro methylation of recombinant or native proteins.

Testing

Validated by in vitro methylation using CH3 Arginine-Rich Peptide.

Storage

4° C

Weight14.4 lbs
Dimensions11 × 9 × 14 in

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CH3 Enzymes
20 mM Tris pH 8