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PC12 Rat Pheochromocytoma Cell Line
$600.00
PC12 cells are embryologically, histologically, and biochemically equivalent to adrenal chromaffin cells. In addition, since the isolation of PC12 cells in the 1970s, these cells have served as a highly regarded model of sympathetic neuron differentiation. When treated with nanogram quantities of nerve growth factor, the cells can survive in the absence of serum, exit the cell cycle, and extend long branched neurites similar to the axon and dendrites of autonomic sympathetic neurons.
- Pheochromocytoma: Adrenal chromaffin cell tumor
- Nerve Growth Factor The first neurotrophin ever discovered is capable of activating specific cell surface receptors on sympathetic neurons to stimulate cell survival and differentiation
PC12 Cell Line
Rat Pheochromocytoma
Product: #91A001
Overview
Rat pheochromocytoma clonal cell line. The original parental line isolated in 1976 by Greene and Tischler (https://www.ncbi.nlm.nih.gov/pubmed/1065897). Neuronal differentiation induced by nerve growth factor.
Re-sale or other commercial use of CH3 PC12 Cell Lines requires the execution of a Licensing Agreement, Materials Transfer Agreement, or other codified accord between CH3 BioSystems LLC and the commercial, for profit organization.
Purification
N/A
Formulation
10 to 20 X 106 cells in flask containing recommended culture medium or frozen cell pellet in cryoprotectant.
Product Usage
Cell culture. Use RPMI 1640 medium; 10% Horse Serum; 5% Fetal Bovine Serum. For neuronal differentiation, use 1% Horse Serum + 50 ng/ml nerve growth factor.
Testing
Proliferation (4 day doubling time) and differentiation tested on a substrate of rat-tail collagen.
Storage
Place in culture immediately upon arrival; 35° humidified CO2 incubator. Use 5 to 7.5% CO2.
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