pLuc-1221neo, human survivin promoter (1221 bp) -luciferase construct (with neomycin gene for G418 selection as an option)
A luciferase reporter construct based upon a plasmid backbone similar to pGEM3 with human survivin promoter
Standard mini-preparation from Amp-r culture.
Transfection of mammalian cells using the method of choice of the laboratory investigator.
Store plasmid DNA at -20°C to -80°C or bacterial cells at -80°C until expansion of transformed bacteria for scaled-up plasmid preparation of vector DNA prior to transfection studies.