Convenient ready-to-use protease inhibitor compatible with in vitro arginine methyltransferase reactions. Dilute 5 μl/ml of assay buffer prior to addition of S-Adenosylmethionine, enzyme and substrate for methyltransferase assay.
Prepared as a 2 mM solution in de-ionized water (≥18 MΩcm).
For use during in vitro methylation of recombinant or native proteins.
Validated by in vitro methylation using CH3 Arginine-Rich Peptide.
Thaw one tube for single use only. Store remaining aliquots at ≤ -20°. Stable for at least 1 year after the date of preparation printed on the label.