HepG2 Cell Line
Hepatoma G2, Human hepatocellular carcinoma
Human hepatocellular carcinoma cell line.
25 to 50 X 106 cells in flask containing RPMI 1640 culture medium and 5% fetal bovine serum or frozen cell pellet in cryoprotectant.
Cell culture. Useful for large-scale cell culture to provide cell proteins for purification in downstream applications. Adapt well to a wide variety of culture media. Fetal bovine serum at 5% is recommended.
Cultured on tissue culture plastic in 5% fetal bovine serum with either DMEM or RPMI 1640.
Place in culture immediately upon arrival; 35° humidified CO2 incubator. Use 5 to 7.5% CO2.