HEK293 Cell Line
Human Embryonic Kidney
Adenovirus‐transformed human embryonic kidney cell clonal line.
25 to 50 X 106 cells in flask containing RPMI 1640 culture medium and 5% fetal bovine serum or frozen cell pellet in cryoprotectant.
Cell culture. Useful for high efficiency transfection of genes and the study of the proteins that are expressed as a result of the transfection. The cells adapt well to a wide variety of culture media. Fetal bovine serum at 5% is recommended.
Cultured on tissue culture plastic in 5% fetal bovine serum with either DMEM or RPMI 1640.
Place in culture immediately upon arrival; 35° humidified CO2 incubator. Use 5 to 7.5% CO2.