Using E0771 Cells for Allograft Transplantation

After removing E0771 cells from cell culture using sterile technique, the cells are counted and injected into a healthy mouse via the mammary pads (typically the fourth mammary pad) or intravenously in the tail vein [1-8]. To see the full effects of tumor growth, 1 x 105 to 1 x 106 must be used for injection. Cells are injected when the mice reach 7-8 weeks old [9].

Primary tumors begin to form within days but must grow for about two weeks before observing metastasis [9].

When you order E0771 cells, CH3 Biosystems always provides 10 to 20 x 106 cells in the vial or flask that is shipped to you. This makes it easier to get your experiment up and running without taking extra time to grow the cells!

(See our video on our homepage!)

 – Written by Nicole Cavanaugh

 

Works Cited

  1. Kitamura, T. et al.(2015). CCL2-induced chemokine cascade promotes breast cancer metastasis by enhancing retention of metastasis-associated macrophages. Journal of Experimental Medicine, 212(7). 
  2. Hoves, S. et al. (2018). Rapid activation of tumor-associated macrophages boosts preexisting tumor immunity. Journal of Experimental Medicine, 215(3). 
  3. Viant, C. et al. (2014). Cell cycle progression dictates the requirement for BCL2 in natural killer cell survival. Journal of Experimental Medicine, 214(2). 
  4. Qian, B. et al. (2015). FLT1 signaling in metastasis-associated macrophages activates an inflammatory signature that promotes breast cancer metastasis. Journal of Experimental Medicine, 212(9).
  5. Ewens, A. et al. (2005). Distant Metastasis from Subcutaneously Grown E0771 Medullary Breast Adenocarcinoma. International Journal of Cancer Research and Treatment, 25(6B). 
  6. Jeon, B. et al. (2018). Actin stabilizer TAGLN2 potentiates adoptive T cell therapy by boosting the inside-out costimulation via lymphocyte function-associated antigen-1. OncoImmunology, 7(12). 
  7. Mariotto, E. et al. (2018). Choline Kinase Alpha Inhibition by EB-3D Triggers Cellular Senescence, Reduces Tumor Growth and Metastatic Dissemination in Breast Cancer. Cancers, 10(10). 
  8. Ouarné, M. et al. (2018). BMP9, but not BMP10, acts as a quiescence factor on tumor growth, vessel normalization and metastasis in a mouse model of breast cancer. Journal of Experimental & Clinical Cancer Research, 37(209). 
  9. Qian, B. et al. (2015). FLT1 signaling in metastasis-associated macrophages activates an inflammatory signature that promotes breast cancer metastasis. Journal of Experimental Medicine, 212(9)