John AlettaJohn Aletta

Research Review Series (2 of 3)

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THE INTERSECTION OF CANCER AND PROTEIN METHYLATION   William Pratt, CH3 Biosystems Lab Intern Introduction   Protein arginine methyltransferases (PRMTs) are transcriptional coactivators, a type of coregulator that affects a transcription factor to increase the rate of transcription of a gene by recruiting general transcription machinery to the promoter region. The aberrant expression of PRMTs … Read more

Research Review Series (1 of 3)

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PRMT5 and Cancer  William Pratt, CH3 Biosystems Lab Intern   Protein methylation is a common post translational modification that has grown in importance as a research topic in recent years. The role of protein methylation in cancer is extremely complicated and thus very difficult to discuss broadly. This series of posts focuses on the relationship … Read more

Product Spotlight

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PC12 Pheochromocytoma Cells     Advantage of PC12 Cells Every year thousands of people in the world are diagnosed with neurodevelopmental diseases and cancer. The need for better and more effective medications that can be brought to the market faster is on the rise. Each new drug is created from years of research and experiments … Read more

Research Spotlight

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Methyltransferase Inhibitors Recent exciting news in the field of personalized medicine of cancer patients: new developments on the collaboration between Epizyme and GlaxoSmithKline may contribute the advancement of cancer treatment research tremendously.  Additionally, there has been new progress made by CH3 BioSystems to create a new drug discovery platform. New development on the collaboration between … Read more

Product Spotlight

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Cell Lines Cell lines are derived from normal or diseased tissue that is disaggregated and subjected to limited dilution to generate one or a few cells. Therefore, after several dozen rounds of division, cultures of cell line consist of cells with uniform genetic make-up and additionally homogenous biochemical composition. Here at CH3 BioSystems, our cell lines are … Read more

Arginine methylation of RUNX1 affects peripheral CD4+ T cells in intact mice

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Arginine methylation of RUNX1 affects peripheral CD4+ T cells in intact mice RUNX1 (previously termed AML1) is a frequent target of human leukaemia-associated gene aberrations, and it encodes the DNA-binding subunit of the Core-Binding Factor transcription factor complex. RUNX1 expression is essential for the initiation of definitive haematopoiesis, for steady-state thrombopoiesis, and for normal lymphocytes … Read more

NF kappa B joins other signaling molecules modified by methylation

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NF kappa B joins other signaling molecules modified by methylation In normal cells exposed to stress, the central transcription factor NF-κB is activated only transiently, to modulate the activation of downstream immune responses. However, in most cancers, NF-κB is abnormally activated constitutively, contributing thus to oncogenesis and tumor progression. Therefore, downregulating NF-κB activity is an … Read more

New Review of transcription factors and methylation

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New Review of transcription factors and methylation Methylation of lysine and arginine residues on histones has long been known to determine both chromatin structure and gene expression. In recent years, the methylation of non-histone proteins has emerged as a prevalent modification which impacts on diverse processes such as cell cycle control, DNA repair, senescence, differentiation, … Read more

PRMT1 regulates cocaine actions in brain

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PRMT1 regulates cocaine actions in brain Recent evidence suggests that histone modifications play a role in the behavioral effects of cocaine in rodent models. Histone arginine is known to be methylated by protein arginine N-methyltransferases (PRMTs). Evidence shows that PRMT1 contributes to >90% of cellular PRMT activity, which regulates histone H4 arginine 3 asymmetric dimethylation … Read more